OrangerV1, Main, Exploration, bibRecord, 002468

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Identifieur interne : 002468 ( Main/Exploration ); précédent : 002467; suivant : 002469

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Auteurs : Alida Ballester [Espagne] ; Magdalena Cervera [Espagne] ; Leandro Pena [Espagne]

Source :

Plant cell reports : (Print) [ 0721-7714 ] ; 2007.

RBID : Pascal:07-0285340

Descripteurs français

Pascal (Inist)
- Production, Plante transgénique, Transferases, Sélection, Gène, Citrus, Site spécifique, Recombinaison, Transformation génétique, Végétal, Citrus sinensis Poncirus trifoliata, Isopentenyl transferase.
Wicri :
- topic : Production, Plante transgénique.

English descriptors

KwdEn :
- Alkyl and Aryl Transferases (genetics), Alkyl and Aryl Transferases (metabolism), Base Sequence, Citrus, Citrus (genetics), Citrus (growth & development), Citrus sinensis (genetics), Citrus sinensis (growth & development), Gene, Genetic Markers, Genetic Vectors (genetics), Genetic transformation, Molecular Sequence Data, Plant Shoots (genetics), Plant Shoots (growth & development), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Production, Recombinases (genetics), Recombinases (metabolism), Recombination, Recombination, Genetic (genetics), Selection, Site specificity, Transferases, Transformation, Genetic, Transgenic plant, Vegetals.
MESH :
- chemical , genetics : Alkyl and Aryl Transferases, Recombinases.
- chemical , metabolism : Alkyl and Aryl Transferases, Recombinases.
- genetics : Citrus, Citrus sinensis, Genetic Vectors, Plant Shoots, Plants, Genetically Modified, Recombination, Genetic.
- growth & development : Citrus, Citrus sinensis, Plant Shoots, Plants, Genetically Modified.
- Base Sequence, Genetic Markers, Molecular Sequence Data, Transformation, Genetic.

Abstract

The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.

Affiliations:

Espagne

Le document en format XML

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<term>Citrus (genetics)</term>
<term>Citrus (growth & development)</term>
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<term>Citrus sinensis (growth & development)</term>
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<term>Genetic Markers</term>
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<front><div type="abstract" xml:lang="en">The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.</div>
</front>
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Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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